Proteomic analysis of pancreatic zymogen granules: identification of new granule proteins.
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ABSTRACT:
SUBMITTER: Rindler MJ
PROVIDER: S-EPMC2582026 | biostudies-literature | 2007 Aug
REPOSITORIES: biostudies-literature
Publications
Proteomic analysis of pancreatic zymogen granules: identification of new granule proteins.
Rindler Michael J MJ Xu Chong-Feng CF Gumper Iwona I Smith Nora N NN Neubert Thomas A TA
Journal of proteome research 20070621 8
The composition of zymogen granules from rat pancreas was determined by LC-MS/MS. Enriched intragranular content, peripheral membrane, and integral membrane protein fractions were analyzed after one-dimensional SDS-PAGE and tryptic digestion of gel slices. A total of 371 proteins was identified with high confidence, including 84 previously identified granule proteins. The 287 remaining proteins included 37 GTP-binding proteins and effectors, 8 tetraspan membrane proteins, and 22 channels and tra ...[more]
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