Unknown

Dataset Information

0

Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet.


ABSTRACT: Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytidine modification. The modification contributes to the tRNA's anticodon domain structure, thermodynamic properties and its ability to bind codons AUA and AUG in translational initiation and elongation.

SUBMITTER: Lusic H 

PROVIDER: S-EPMC2582600 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Synthesis and investigation of the 5-formylcytidine modified, anticodon stem and loop of the human mitochondrial tRNAMet.

Lusic Hrvoje H   Gustilo Estella M EM   Vendeix Franck A P FA   Kaiser Rob R   Delaney Michael O MO   Graham William D WD   Moye Virginia A VA   Cantara William A WA   Agris Paul F PF   Deiters Alexander A  

Nucleic acids research 20081016 20


Human mitochondrial methionine transfer RNA (hmtRNA(Met)(CAU)) has a unique post-transcriptional modification, 5-formylcytidine, at the wobble position-34 (f(5)C(34)). The role of this modification in (hmtRNA(Met)(CAU)) for the decoding of AUA, as well as AUG, in both the peptidyl- and aminoacyl-sites of the ribosome in either chain initiation or chain elongation is still unknown. We report the first synthesis and analyses of the tRNA's anticodon stem and loop domain containing the 5-formylcytid  ...[more]

Similar Datasets

| S-EPMC1322268 | biostudies-literature
| S-EPMC2655697 | biostudies-literature
| S-EPMC6462517 | biostudies-literature
| S-EPMC2528185 | biostudies-literature
| S-EPMC3162475 | biostudies-literature
2021-11-17 | GSE183923 | GEO
| S-EPMC7822408 | biostudies-literature
| S-EPMC3198592 | biostudies-literature
| S-EPMC145695 | biostudies-other
2023-06-07 | GSE230043 | GEO