Ontology highlight
ABSTRACT:
SUBMITTER: Soifer HS
PROVIDER: S-EPMC2582626 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Soifer Harris S HS Sano Masayuki M Sakurai Kumi K Chomchan Pritsana P Saetrom Pål P Sherman Mark A MA Collingwood Michael A MA Behlke Mark A MA Rossi John J JJ
Nucleic acids research 20081015 20
In humans a single species of the RNAseIII enzyme Dicer processes both microRNA precursors into miRNAs and long double-stranded RNAs into small interfering RNAs (siRNAs). An interesting but poorly understood domain of the mammalian Dicer protein is the N-terminal helicase-like domain that possesses a signature DExH motif. Cummins et al. created a human Dicer mutant cell line by inserting an AAV targeting cassette into the helicase domain of both Dicer alleles in HCT116 cells generating an in-fra ...[more]