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Brucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression.


ABSTRACT: To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies.

SUBMITTER: Rajasekaran P 

PROVIDER: S-EPMC2583484 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Brucella abortus strain RB51 leucine auxotroph as an environmentally safe vaccine for plasmid maintenance and antigen overexpression.

Rajasekaran Parthiban P   Seleem Mohamed N MN   Contreras Andrea A   Purwantini Endang E   Schurig Gerhardt G GG   Sriranganathan Nammalwar N   Boyle Stephen M SM  

Applied and environmental microbiology 20081003 22


To avoid potentiating the spread of an antibiotic resistance marker, a plasmid expressing a leuB gene and a heterologous antigen, green fluorescent protein (GFP), was shown to complement a leucine auxotroph of cattle vaccine strain Brucella abortus RB51, which protected CD1 mice from virulent B. abortus 2308 and elicited GFP antibodies. ...[more]

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