Ontology highlight
ABSTRACT:
SUBMITTER: Liu CC
PROVIDER: S-EPMC2584696 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Liu Chang C CC Mack Antha V AV Tsao Meng-Lin ML Mills Jeremy H JH Lee Hyun Soo HS Choe Hyeryun H Farzan Michael M Schultz Peter G PG Smider Vaughn V VV
Proceedings of the National Academy of Sciences of the United States of America 20081111 46
We have devised a phage display system in which an expanded genetic code is available for directed evolution. This system allows selection to yield proteins containing unnatural amino acids should such sequences functionally outperform ones containing only the 20 canonical amino acids. We have optimized this system for use with several unnatural amino acids and provide a demonstration of its utility through the selection of anti-gp120 antibodies. One such phage-displayed antibody, selected from ...[more]