Ontology highlight
ABSTRACT:
SUBMITTER: Marriott G
PROVIDER: S-EPMC2584753 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Marriott Gerard G Mao Shu S Sakata Tomoyo T Ran Jing J Jackson David K DK Petchprayoon Chutima C Gomez Timothy J TJ Warp Erica E Tulyathan Orapim O Aaron Holly L HL Isacoff Ehud Y EY Yan Yuling Y
Proceedings of the National Academy of Sciences of the United States of America 20081112 46
One of the limitations on imaging fluorescent proteins within living cells is that they are usually present in small numbers and need to be detected over a large background. We have developed the means to isolate specific fluorescence signals from background by using lock-in detection of the modulated fluorescence of a class of optical probe termed "optical switches." This optical lock-in detection (OLID) approach involves modulating the fluorescence emission of the probe through deterministic, ...[more]