Ontology highlight
ABSTRACT:
SUBMITTER: Subach OM
PROVIDER: S-EPMC2585067 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Subach Oksana M OM Gundorov Illia S IS Yoshimura Masami M Subach Fedor V FV Zhang Jinghang J Grüenwald David D Souslova Ekaterina A EA Chudakov Dmitriy M DM Verkhusha Vladislav V VV
Chemistry & biology 20081001 10
We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. The strategy was applied to red fluorescent proteins of the different genetic backgrounds, such as TagRFP, mCherry, HcRed1, M355NA, and mKeima, which all were converted into blue probes. Further improvement of the blue variant of TagRFP by random mutagenesis res ...[more]