Ricin B chain targeted to the endoplasmic reticulum of tobacco protoplasts is degraded by a CDC48- and vacuole-independent mechanism.
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ABSTRACT:
SUBMITTER: Chamberlain KL
PROVIDER: S-EPMC2586253 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
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Ricin B chain targeted to the endoplasmic reticulum of tobacco protoplasts is degraded by a CDC48- and vacuole-independent mechanism.
Chamberlain Kerry L KL Marshall Richard S RS Jolliffe Nicholas A NA Frigerio Lorenzo L Ceriotti Aldo A Lord J Michael JM Roberts Lynne M LM
The Journal of biological chemistry 20081002 48
The B chain of ricin was expressed and delivered to the endoplasmic reticulum of tobacco protoplasts where it disappeared with time in a manner consistent with degradation. This turnover did not occur in the vacuoles or upon secretion. Indeed, several lines of evidence indicate that, in contrast to the turnover of endoplasmic reticulum-targeted ricin A chain in the cytosol, the bulk of expressed ricin B chain was degraded in the secretory pathway. ...[more]
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