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Nanofluidic biosensing for beta-amyloid detection using surface enhanced Raman spectroscopy.

ABSTRACT: Trace detection of the conformational transition of beta-amyloid peptide (Abeta) from a predominantly alpha-helical structure to beta-sheet could have a large impact in understanding and diagnosing Alzheimer's disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Abeta in different conformational states during the Abeta self-assembly process as well as to distinguish Abeta from confounder proteins commonly found in cerebral spinal fluid.

SUBMITTER: Chou IH

PROVIDER: S-EPMC2587305 | biostudies-literature | 2008 Jun

REPOSITORIES: biostudies-literature

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Nanofluidic biosensing for beta-amyloid detection using surface enhanced Raman spectroscopy.

Chou I-Hsien IH Benford Melodie M Beier Hope T HT Coté Gerard L GL Wang Miao M Jing Nan N Kameoka Jun J Good Theresa A TA

Nano letters 20080520 6

Trace detection of the conformational transition of beta-amyloid peptide (Abeta) from a predominantly alpha-helical structure to beta-sheet could have a large impact in understanding and diagnosing Alzheimer's disease. We demonstrate how a novel nanofluidic biosensor using a controlled, reproducible surface enhanced Raman spectroscopy active site was developed to observe Abeta in different conformational states during the Abeta self-assembly process as well as to distinguish Abeta from confounde ...[more]

PMID: 18489171

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