Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize.
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ABSTRACT:
SUBMITTER: Zhu C
PROVIDER: S-EPMC2587607 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
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Combinatorial genetic transformation generates a library of metabolic phenotypes for the carotenoid pathway in maize.
Zhu Changfu C Naqvi Shaista S Breitenbach Jürgen J Sandmann Gerhard G Christou Paul P Capell Teresa T
Proceedings of the National Academy of Sciences of the United States of America 20081114 47
Combinatorial nuclear transformation is a novel method for the rapid production of multiplex-transgenic plants, which we have used to dissect and modify a complex metabolic pathway. To demonstrate the principle, we transferred 5 carotenogenic genes controlled by different endosperm-specific promoters into a white maize variety deficient for endosperm carotenoid synthesis. We recovered a diverse population of transgenic plants expressing different enzyme combinations and showing distinct metaboli ...[more]
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