Dissecting the cell entry pathway of dengue virus by single-particle tracking in living cells.
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ABSTRACT:
SUBMITTER: van der Schaar HM
PROVIDER: S-EPMC2592694 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Publications
Dissecting the cell entry pathway of dengue virus by single-particle tracking in living cells.
van der Schaar Hilde M HM Rust Michael J MJ Chen Chen C van der Ende-Metselaar Heidi H Wilschut Jan J Zhuang Xiaowei X Smit Jolanda M JM
PLoS pathogens 20081219 12
Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and single-virus tracking to investigate the cell entry, endocytic trafficking, and fusion behavior of DENV. Simultaneous tracking of DENV particles and various endocytic markers revealed that DENV enters cells exclusively via clathrin-mediated endocytosis. The virus particles move ...[more]
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