Experimental evidence for splicing of intron-containing transcripts of plant LTR retrotransposon Ogre.

ABSTRACT: Ogre elements are a distinct group of plant Ty3/gypsy-like retrotransposons characterized by several specific features, one of which is a separation of the gag-pol region into two non-overlapping open reading frames: ORF2 coding for Gag-Pro, and ORF3 coding for RT/RH-INT proteins. Previous characterization of Ogre elements from several plant species revealed that part of their transcripts lacks the region between ORF2 and ORF3, carrying one uninterrupted ORF instead. In this work, we investigated a hypothesis that this region represents an intron that is spliced out from part of the Ogre transcripts as a means for preferential production of ORF2-encoded proteins over those encoded by the complete ORF2-ORF3 region. The experiments involved analysis of transcription patterns of well-defined

SUBMITTER: Steinbauerova V 

PROVIDER: S-EPMC2596294 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature