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Deficit of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats.

ABSTRACT: Epileptogenesis in mesial temporal lobe epilepsy is determined by several factors including abnormalities in the expression and function of ion channels. Here, we report a long-lasting deficit in gene expression of Kcnma1 coding for the large-conductance calcium-activated potassium (BK, MaxiK) channel alpha-subunits after pilocarpine-induced status epilepticus. By using comparative real-time PCR, Taqman gene expression assays, and the delta-delta comparative threshold method we detected a significant reduction in Kcnma1 expression in microdissected dentate gyrus at different intervals after status epilepticus (24 h, 10 days, 1 month, and more than 2 months). BK channels are key regulators of neuronal excitability and transmitter release. Hence, defective Kcnma1 expression may play a critical role in the pathogenesis of mesial temporal lobe epilepsy.

SUBMITTER: Ermolinsky B

PROVIDER: S-EPMC2596301 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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Deficit of Kcnma1 mRNA expression in the dentate gyrus of epileptic rats.

Ermolinsky Boris B Arshadmansab Massoud F MF Pacheco Otalora Luis F LF Zarei Masoud M MM Garrido-Sanabria Emilio R ER

Neuroreport 20080801 13

Epileptogenesis in mesial temporal lobe epilepsy is determined by several factors including abnormalities in the expression and function of ion channels. Here, we report a long-lasting deficit in gene expression of Kcnma1 coding for the large-conductance calcium-activated potassium (BK, MaxiK) channel alpha-subunits after pilocarpine-induced status epilepticus. By using comparative real-time PCR, Taqman gene expression assays, and the delta-delta comparative threshold method we detected a signif ...[more]

PMID: 18695509

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