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Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope.

ABSTRACT: We present a handheld dual-axes confocal microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 microm x 260 microm. The transverse and axial resolutions of the handheld probe are 1.7 microm and 5.8 microm, respectively. Capability to perform real time small animal imaging is demonstrated in vivo in transgenic mice.

SUBMITTER: Ra H

PROVIDER: S-EPMC2596951 | biostudies-literature | 2008 May

REPOSITORIES: biostudies-literature

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Three-dimensional in vivo imaging by a handheld dual-axes confocal microscope.

Ra Hyejun H Piyawattanametha Wibool W Mandella Michael J MJ Hsiung Pei-Lin PL Hardy Jonathan J Wang Thomas D TD Contag Christopher H CH Kino Gordon S GS Solgaard Olav O

Optics express 20080501 10

We present a handheld dual-axes confocal microscope that is based on a two-dimensional microelectromechanical systems (MEMS) scanner. It performs reflectance and fluorescence imaging at 488 nm wavelength, with three-dimensional imaging capability. The fully packaged microscope has a diameter of 10 mm and acquires images at 4 Hz frame rate with a maximum field of view of 400 microm x 260 microm. The transverse and axial resolutions of the handheld probe are 1.7 microm and 5.8 microm, respectively ...[more]

PMID: 18545427

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