Mechanism for coordinated RNA packaging and genome replication by rotavirus polymerase VP1.
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ABSTRACT:
SUBMITTER: Lu X
PROVIDER: S-EPMC2602806 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Publications
Mechanism for coordinated RNA packaging and genome replication by rotavirus polymerase VP1.
Lu Xiaohui X McDonald Sarah M SM Tortorici M Alejandra MA Tao Yizhi Jane YJ Vasquez-Del Carpio Rodrigo R Nibert Max L ML Patton John T JT Harrison Stephen C SC
Structure (London, England : 1993) 20081101 11
Rotavirus RNA-dependent RNA polymerase VP1 catalyzes RNA synthesis within a subviral particle. This activity depends on core shell protein VP2. A conserved sequence at the 3' end of plus-strand RNA templates is important for polymerase association and genome replication. We have determined the structure of VP1 at 2.9 A resolution, as apoenzyme and in complex with RNA. The cage-like enzyme is similar to reovirus lambda3, with four tunnels leading to or from a central, catalytic cavity. A distingu ...[more]
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