Ontology highlight
ABSTRACT:
SUBMITTER: Kinsley N
PROVIDER: S-EPMC2603631 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Kinsley Nichole N Sayed Yasien Y Mosebi Salerwe S Armstrong Richard N RN Dirr Heini W HW
Biophysical chemistry 20080805 2-3
Molecular docking and ANS-displacement experiments indicated that 8-anilinonaphthalene sulfonate (ANS) binds the hydrophobic site (H-site) in the active site of dimeric class Mu rGST M1-1. The naphthalene moiety provides most of the van der Waals contacts at the ANS-binding interface while the anilino group is able to sample different rotamers. The energetics of ANS binding were studied by isothermal titration calorimetry (ITC) over the temperature range of 5-30 degrees C. Binding is both enthal ...[more]