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Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition.

ABSTRACT: Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between longissimus dorsi (LD) muscles with extremely different IMF content. A major DE gene associated with IMF content was identified as splicing factor serine-arginine rich protein (SFRS18) gene, also known as SRrp130. The gene exhibited relatively higher expression levels in LD muscles with higher IMF content. A full-length cDNA sequence of pig SFRS18 gene was obtained by in silico comparative cloning coupled with PCR target sequencing, while the current EST (expressed sequence tag) database supported two transcript variants of the pig gene. Differential expression of the SFRS18 gene was further confirmed using quantitative PCR. The mRNA levels of SFRS18 gene showed significant and positive correlation with IMF content in LD muscle (r = 0.54, P < 0.01). Collectively, these results suggest that the SFRS18 gene is involved in the regulation of IMF deposition in pig and that it may be a useful tool in selecting animals for desired amounts of fatness for high quality pork.

SUBMITTER: Wang X

PROVIDER: S-EPMC2605576 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition.

Wang Xiuxing X Xue Chunyang C Wang Xiaona X Liu Honglin H Xu Yinxue Y Zhao Ruqian R Jiang Zhihua Z Dodson Michael V MV Chen Jie J

International journal of biological sciences 20081217 1

Intramuscular fat (IMF) content plays a key role in establishing pork quality. In the present study, differential-display reverse transcription-polymerase chain reaction (DDRT-PCR) was used to identify differentially expressed (DE) genes between longissimus dorsi (LD) muscles with extremely different IMF content. A major DE gene associated with IMF content was identified as splicing factor serine-arginine rich protein (SFRS18) gene, also known as SRrp130. The gene exhibited relatively higher exp ...[more]

PMID: 19119313

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Project description:BACKGROUND: Intramuscular fat (IMF) is one of the important factors influencing meat quality, however, for chickens, the molecular regulatory mechanisms underlying this trait have not yet been determined. In this study, a systematic identification of candidate genes and new pathways related to IMF deposition in chicken breast tissue has been made using gene expression profiles of two distinct breeds: Beijing-you (BJY), a slow-growing Chinese breed possessing high meat quality and Arbor Acres (AA), a commercial fast-growing broiler line. RESULTS: Agilent cDNA microarray analyses were conducted to determine gene expression profiles of breast muscle sampled at different developmental stages of BJY and AA chickens. Relative to d 1 when there is no detectable IMF, breast muscle at d 21, d 42, d 90 and d 120 (only for BJY) contained 1310 differentially expressed genes (DEGs) in BJY and 1080 DEGs in AA. Of these, 34-70 DEGs related to lipid metabolism or muscle development processes were examined further in each breed based on Gene Ontology (GO) analysis. The expression of several DEGs was correlated, positively or negatively, with the changing patterns of lipid content or breast weight across the ages sampled, indicating that those genes may play key roles in these developmental processes. In addition, based on KEGG pathway analysis of DEGs in both BJY and AA chickens, it was found that in addition to pathways affecting lipid metabolism (pathways for MAPK & PPAR signaling), cell junction-related pathways (tight junction, ECM-receptor interaction, focal adhesion, regulation of actin cytoskeleton), which play a prominent role in maintaining the integrity of tissues, could contribute to the IMF deposition. CONCLUSION: The results of this study identified potential candidate genes associated with chicken IMF deposition and imply that IMF deposition in chicken breast muscle is regulated and mediated not only by genes and pathways related to lipid metabolism and muscle development, but also by others involved in cell junctions. These findings establish the groundwork and provide new clues for deciphering the molecular mechanisms underlying IMF deposition in poultry. Further studies at the translational and posttranslational level are now required to validate the genes and pathways identified here.

Project description:BACKGROUND:The Intramuscular fat (IMF) content in meat products, which is positively correlated with meat quality, is an important trait considered by consumers. The regulation of IMF deposition is species specific. However, the IMF-deposition-related mRNA and non-coding RNA and their regulatory network in yak (Bos grunniens) remain unknown. High-throughput sequencing technology provides a powerful approach for analyzing the association between transcriptome-related differences and specific traits in animals. Thus, the whole transcriptomes of yak muscle and adipose tissues were screened and analyzed to elucidate the IMF deposition-related genes. The muscle tissues were used for IMF content measurements. RESULTS:Significant differences were observed between the 0.5- and 2.5-year-old yaks. Several mRNAs, miRNAs, lncRNAs and circRNAs were generally expressed in both muscle and adipose tissues. Between the 0.5- and 2.5-year-old yaks, 149 mRNAs, 62 miRNAs, 4 lncRNAs, and 223 circRNAs were differentially expressed in muscle tissue, and 72 mRNAs, 15 miRNAs, 9 lncRNAs, and 211 circRNAs were differentially expressed in adipose tissue. KEGG annotation revelved that these differentially expressed genes were related to pathways that maintain normal biological functions of muscle and adipose tissues. Moreover, 16 mRNAs, 5 miRNAs, 3 lncRNAs, and 5 circRNAs were co-differentially expressed in both types of tissue. We suspected that these co-differentially expressed genes were involved in IMF-deposition in the yak. Additionally, LPL, ACADL, SCD, and FASN, which were previously shown to be associated with the IMF content, were identified in the competing endogenous RNA (ceRNA) regulatory network that was constructed on the basis of the IMF deposition-related genes. Three ceRNA subnetworks also revealed that TCONS-00016416 and its target SIRT1 "talk" to each other through the same miR-381-y and miR-208 response elements, whereas TCONS-00061798 and its target PRKCA, and TCONS-00084092 and its target LPL "talk" to each other through miR-122-x and miR-499-y response elements, respectively. CONCLUSION:Taken together, our results reveal the potential mRNA and noncoding RNAs involved in IMF deposition in the yak, providing a useful resource for further research on IMF deposition in this animal species.

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Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition.

Publications

Differential display of expressed genes reveals a novel function of SFRS18 in regulation of intramuscular fat deposition.

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