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Interaction of onconase with the human ribonuclease inhibitor protein.


ABSTRACT: One of the tightest known protein-protein interactions in biology is that between members of the ribonuclease A superfamily and the ribonuclease inhibitor protein (RI). Some members of this superfamily are able to kill cancer cells, and the ability to evade RI is a major determinant of whether a ribonuclease will be cytotoxic. The archetypal cytotoxic ribonuclease, onconase (ONC), is in late-stage clinical trials for the treatment of malignant mesothelioma. We present here the first measurement of the inhibition of the ribonucleolytic activity of ONC by RI. This inhibition occurs with K(i)=0.15muM in a solution of low salt concentration.

SUBMITTER: Turcotte RF 

PROVIDER: S-EPMC2605674 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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Interaction of onconase with the human ribonuclease inhibitor protein.

Turcotte Rebecca F RF   Raines Ronald T RT  

Biochemical and biophysical research communications 20081016 2


One of the tightest known protein-protein interactions in biology is that between members of the ribonuclease A superfamily and the ribonuclease inhibitor protein (RI). Some members of this superfamily are able to kill cancer cells, and the ability to evade RI is a major determinant of whether a ribonuclease will be cytotoxic. The archetypal cytotoxic ribonuclease, onconase (ONC), is in late-stage clinical trials for the treatment of malignant mesothelioma. We present here the first measurement  ...[more]

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