Ontology highlight
ABSTRACT:
SUBMITTER: Babic AC
PROVIDER: S-EPMC2605692 | biostudies-literature | 2008 Oct
REPOSITORIES: biostudies-literature
Babic Andrea C AC Little Elizabeth J EJ Manohar Veena M VM Bitinaite Jurate J Horton Nancy C NC
Journal of molecular biology 20080822 1
Five new structures of the Q138F HincII enzyme bound to a total of three different DNA sequences and three different metal ions (Ca(2+), Mg(2+), and Mn(2+)) are presented. While previous structures were produced from soaking Ca(2+) into preformed Q138F HincII/DNA crystals, the new structures are derived from cocrystallization with Ca(2+), Mg(2+), or Mn(2+). The Mn(2)(+)-bound structure provides the first view of a product complex of Q138F HincII with cleaved DNA. Binding studies and a crystal st ...[more]