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Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance.

ABSTRACT: The genes encoding Rev1 and DNA polymerase zeta (Rev3/Rev7) are together required for the vast majority of DNA damage-induced mutations in eukaryotes from yeast to humans. Here, we provide insight into the critical role that the Saccharomyces cerevisiae Rev1 C-terminus plays in the process of mutagenic DNA damage tolerance. The Rev1 C-terminus was previously thought to be poorly conserved and therefore not likely to be important for mediating protein-protein interactions. However, through comprehensive alignments of the Rev1 C-terminus, we have identified novel and hitherto unrecognized conserved motifs that we show play an essential role in REV1-dependent survival and mutagenesis in S. cerevisiae, likely in its post-replicative gap-filling mode. We further show that the minimal C-terminal fragment of Rev1 containing these highly conserved motifs is sufficient to interact with Rev7.

SUBMITTER: D'Souza S

PROVIDER: S-EPMC2606931 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature

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Novel conserved motifs in Rev1 C-terminus are required for mutagenic DNA damage tolerance.

D'Souza Sanjay S Waters Lauren S LS Walker Graham C GC

DNA repair 20080707 9

The genes encoding Rev1 and DNA polymerase zeta (Rev3/Rev7) are together required for the vast majority of DNA damage-induced mutations in eukaryotes from yeast to humans. Here, we provide insight into the critical role that the Saccharomyces cerevisiae Rev1 C-terminus plays in the process of mutagenic DNA damage tolerance. The Rev1 C-terminus was previously thought to be poorly conserved and therefore not likely to be important for mediating protein-protein interactions. However, through compre ...[more]

PMID: 18603483

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