Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of Bacillus halodurans.
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ABSTRACT:
SUBMITTER: Berger E
PROVIDER: S-EPMC2612215 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
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Impact of inactivated extracellular proteases on the modified flagellin type III secretion pathway of Bacillus halodurans.
Berger Eldie E du Plessis Erika E Gerber Isak I Crampton Michael M Nxumalo Nolwandle N Louw Maureen M
Applied and environmental microbiology 20081107 1
The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases. ...[more]
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