Ontology highlight
ABSTRACT:
SUBMITTER: Buskiewicz IA
PROVIDER: S-EPMC2612770 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Buskiewicz Iwona A IA Jöckel Johannes J Rodnina Marina V MV Wintermeyer Wolfgang W
RNA (New York, N.Y.) 20081124 1
The bacterial signal recognition particle (SRP) binds to ribosomes synthesizing inner membrane proteins and, by interaction with the SRP receptor, FtsY, targets them to the translocon at the membrane. Here we probe the conformation of SRP and SRP protein, Ffh, at different stages of targeting by measuring fluorescence resonance energy transfer (FRET) between fluorophores placed at various positions within SRP. Distances derived from FRET indicate that SRP binding to nontranslating ribosomes trig ...[more]