Ontology highlight
ABSTRACT:
SUBMITTER: Harvey CD
PROVIDER: S-EPMC2614750 | biostudies-literature | 2008 Dec
REPOSITORIES: biostudies-literature
Harvey Christopher D CD Ehrhardt Anka G AG Cellurale Cristina C Zhong Haining H Yasuda Ryohei R Davis Roger J RJ Svoboda Karel K
Proceedings of the National Academy of Sciences of the United States of America 20081125 49
The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly rep ...[more]