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A genetically encoded fluorescent sensor of ERK activity.


ABSTRACT: The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly reported ERK activation in HEK293 cells after epidermal growth factor stimulation. EKAR signals were correlated with ERK phosphorylation, required ERK activity, and did not report the activities of JNK or p38. EKAR reported ERK activation in the dendrites and nucleus of hippocampal pyramidal neurons in brain slices after theta-burst stimuli or trains of back-propagating action potentials. EKAR therefore permits the measurement of spatiotemporal ERK signaling dynamics in living cells, including in neuronal compartments in intact tissues.

SUBMITTER: Harvey CD 

PROVIDER: S-EPMC2614750 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature

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A genetically encoded fluorescent sensor of ERK activity.

Harvey Christopher D CD   Ehrhardt Anka G AG   Cellurale Cristina C   Zhong Haining H   Yasuda Ryohei R   Davis Roger J RJ   Svoboda Karel K  

Proceedings of the National Academy of Sciences of the United States of America 20081125 49


The activity of the ERK has complex spatial and temporal dynamics that are important for the specificity of downstream effects. However, current biochemical techniques do not allow for the measurement of ERK signaling with fine spatiotemporal resolution. We developed a genetically encoded, FRET-based sensor of ERK activity (the extracellular signal-regulated kinase activity reporter, EKAR), optimized for signal-to-noise ratio and fluorescence lifetime imaging. EKAR selectively and reversibly rep  ...[more]

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