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Development of an ELISA to detect Sin Nombre virus-specific IgM from deer mice (Peromyscus maniculatus).


ABSTRACT: Peromyscus maniculatus (deer mouse) is the primary reservoir for Sin Nombre virus (SNV). Although the presence of IgG antibodies is often used as a marker of infection, it provides little information on active infections in a population but usually is an indicator of past infections. The presence of IgM antibodies is a much better marker for determining whether active infections are present in a population. A mu-capture SNV-specific IgM enzyme linked immunosorbent assay (ELISA) was developed. From live-trap and release studies a total of 68 rodent sera were studied for the presence of Sin Nombre virus-specific IgG and IgM antibodies. In these studies, IgM responses were detected in a number of animals. In some cases early SNV infection was determined through the presence of anti-SNV IgM before IgG antibodies could be detected. From the set of animals analyzed, it was concluded that the IgM response against SNV can persist anywhere from 1 to up to over 2 months, with a median of less than 1 month. Most importantly, it was demonstrated that anti-Sin Nombre virus IgM is an important tool for detection of early infections in rodents and should be considered as a key diagnostic tool.

SUBMITTER: Bego MG 

PROVIDER: S-EPMC2615055 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature

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Development of an ELISA to detect Sin Nombre virus-specific IgM from deer mice (Peromyscus maniculatus).

Bego Mariana G MG   Bawiec Darcy D   Dandge Deepa D   Martino Benjamin B   Dearing Denise D   Wilson Eric E   St Jeor Stephen S  

Journal of virological methods 20080630 2


Peromyscus maniculatus (deer mouse) is the primary reservoir for Sin Nombre virus (SNV). Although the presence of IgG antibodies is often used as a marker of infection, it provides little information on active infections in a population but usually is an indicator of past infections. The presence of IgM antibodies is a much better marker for determining whether active infections are present in a population. A mu-capture SNV-specific IgM enzyme linked immunosorbent assay (ELISA) was developed. Fr  ...[more]

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