Ontology highlight
ABSTRACT:
SUBMITTER: Schaeffer D
PROVIDER: S-EPMC2615074 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Schaeffer Daneen D Tsanova Borislava B Barbas Ana A Reis Filipa Pereira FP Dastidar Eeshita Ghosh EG Sanchez-Rotunno Maya M Arraiano Cecília Maria CM van Hoof Ambro A
Nature structural & molecular biology 20081207 1
The eukaryotic exosome is a ten-subunit 3' exoribonucleolytic complex responsible for many RNA-processing and RNA-degradation reactions. How the exosome accomplishes this is unknown. Rrp44 (also known as Dis3), a member of the RNase II family of enzymes, is the catalytic subunit of the exosome. We show that the PIN domain of Rrp44 has endoribonucleolytic activity. The PIN domain is preferentially active toward RNA with a 5' phosphate, suggesting coordination of 5' and 3' processing. We also show ...[more]