Mutagenic inverted repeat assisted genome engineering (MIRAGE).
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ABSTRACT:
SUBMITTER: Nair NU
PROVIDER: S-EPMC2615605 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Publications
Mutagenic inverted repeat assisted genome engineering (MIRAGE).
Nair Nikhil U NU Zhao Huimin H
Nucleic acids research 20081202 1
Here we describe a one-step method to create precise modifications in the genome of Saccharomyces cerevisiae as a tool for synthetic biology, metabolic engineering, systems biology and genetic studies. Through homologous recombination, a mutagenesis cassette containing an inverted repeat of selection marker(s) is integrated into the genome. Due to its inherent instability in genomic DNA, the inverted repeat catalyzes spontaneous self-excision, resulting in precise genome modification. Since this ...[more]
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