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Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses.


ABSTRACT: The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific parts of the gene encoding phospholipase D in A. haemolyticum. Use of the latter could possibly improve future identification of this generally human pathogenic bacterial species which, according to the present results, seems to occur also in infections of horses.

SUBMITTER: Hassan AA 

PROVIDER: S-EPMC2620868 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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Phenotypic and genotypic characterization of Arcanobacterium haemolyticum isolates from infections of horses.

Hassan Abdulwahed Ahmed AA   Ulbegi-Mohyla Hivda H   Kanbar Talah T   Alber Jörg J   Lämmler Christoph C   Abdulmawjood Amir A   Zschöck Michael M   Weiss Reinhard R  

Journal of clinical microbiology 20081119 1


The present study was designed to characterize phenotypically and genotypically seven Arcanobacterium haemolyticum strains obtained from infections of six horses. All seven strains showed the cultural and biochemical properties typical of A. haemolyticum and were susceptible to most of the antibiotics tested. The species identification could be confirmed by amplification and sequencing of the 16S rRNA gene and the 16S-23S rRNA intergenic spacer region and by PCR amplification of species-specific  ...[more]

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