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Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea.

ABSTRACT: Biomass samples from the Black Sea collected in 1988 were analyzed for SSU genes from Bacteria and Archaea after 10 years of storage at -80 degrees C. Both clonal libraries and direct fingerprinting by terminal restriction fragment length polymorphism (T-RFLP) analyses were used to assess the microbial community. Uniform and discrete depth distributions of different SSU phylotypes were observed. However, most recombinant clones were not restricted to a specific depth in the water column, and many of the major T-RFLP peaks remain uncharacterized. Of the clones obtained, an epsilon-Proteobacteria and a Pseudoalteromonas-like clone accounted for major peaks in the fingerprint, while deeply branching lineages of alpha- and gamma-Proteobacteria were associated with smaller peaks. Additionally, members were found among both the delta-Proteobacteria related to sulfate reducers and the Archaea related to phylotypes from the ANME groups that anaerobically oxidize methane.

SUBMITTER: Vetriani C

PROVIDER: S-EPMC262261 | biostudies-literature | 2003 Nov

REPOSITORIES: biostudies-literature

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Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea.

Vetriani Costantino C Tran Hiep V HV Kerkhof Lee J LJ

Applied and environmental microbiology 20031101 11

Biomass samples from the Black Sea collected in 1988 were analyzed for SSU genes from Bacteria and Archaea after 10 years of storage at -80 degrees C. Both clonal libraries and direct fingerprinting by terminal restriction fragment length polymorphism (T-RFLP) analyses were used to assess the microbial community. Uniform and discrete depth distributions of different SSU phylotypes were observed. However, most recombinant clones were not restricted to a specific depth in the water column, and man ...[more]

PMID: 14602603

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Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea.

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Fingerprinting microbial assemblages from the oxic/anoxic chemocline of the Black Sea.

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