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Role of the stationary growth phase sigma factor RpoS of Burkholderia pseudomallei in response to physiological stress conditions.

ABSTRACT: The Burkholderia pseudomallei rpoS gene was identified, and an rpoS null mutant was constructed. The mutant was shown to have an increased sensitivity to carbon starvation and oxidative stress. By using rpoS-lacZ fusions, transcription of rpoS was shown to be growth phase regulated, reaching a peak upon entry into stationary phase.

SUBMITTER: Subsin B

PROVIDER: S-EPMC262693 | biostudies-literature | 2003 Dec

REPOSITORIES: biostudies-literature

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Role of the stationary growth phase sigma factor RpoS of Burkholderia pseudomallei in response to physiological stress conditions.

Subsin Benchamas B Thomas Mark S MS Katzenmeier Gerd G Shaw Jonathan G JG Tungpradabkul Sumalee S Kunakorn Mongkol M

Journal of bacteriology 20031201 23

The Burkholderia pseudomallei rpoS gene was identified, and an rpoS null mutant was constructed. The mutant was shown to have an increased sensitivity to carbon starvation and oxidative stress. By using rpoS-lacZ fusions, transcription of rpoS was shown to be growth phase regulated, reaching a peak upon entry into stationary phase. ...[more]

PMID: 14617667

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Project description:BackgroundBurkholderia pseudomallei, the causative agent of melioidosis, is a Gram-negative bacterium widely distributed in soil and water in endemic areas. This soil saprophyte can survive harsh environmental conditions, even in soils where herbicides (containing superoxide generators) are abundant. Sigma factor E (σE) is a key regulator of extra-cytoplasmic stress response in Gram-negative bacteria. In this study, we identified the B. pseudomallei σE regulon and characterized the indirect role that σE plays in the regulation of spermidine, contributing to the successful survival of B. pseudomallei in stressful environments.ResultsChanges in the global transcriptional profiles of B. pseudomallei wild type and σE mutant under physiological and oxidative stress (hydrogen peroxide) conditions were determined. We identified 307 up-regulated genes under oxidative stress condition. Comparison of the transcriptional profiles of B. pseudomallei wild type and σE mutant under control or oxidative stress conditions identified 85 oxidative-responsive genes regulated by σE, including genes involved in cell membrane repair, maintenance of protein folding and oxidative stress response and potential virulence factors such as a type VI secretion system (T6SS). Importantly, we identified that the speG gene, encoding spermidine-acetyltransferase, is a novel member of the B. pseudomallei σE regulon. The expression of speG was regulated by σE, implying that σE plays an indirect role in the regulation of physiological level of spermidine to protect the bacteria during oxidative stress.ConclusionThis study identified B. pseudomallei genes directly regulated by σE in response to oxidative stress and revealed the indirect role of σE in the regulation of the polyamine spermidine (via regulation of speG) for bacterial cell protection during oxidative stress. This study provides new insights into the regulatory mechanisms by which σE contributes to the survival of B. pseudomallei under stressful conditions.

Dataset Information

Role of the stationary growth phase sigma factor RpoS of Burkholderia pseudomallei in response to physiological stress conditions.

Publications

Role of the stationary growth phase sigma factor RpoS of Burkholderia pseudomallei in response to physiological stress conditions.

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