Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
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ABSTRACT:
SUBMITTER: Kagawa M
PROVIDER: S-EPMC262715 | biostudies-literature | 2003 Dec
REPOSITORIES: biostudies-literature
Publications
Crystal structure of Bacillus subtilis alpha-amylase in complex with acarbose.
Kagawa Masayuki M Fujimoto Zui Z Momma Mitsuru M Takase Kenji K Mizuno Hiroshi H
Journal of bacteriology 20031201 23
The crystal structure of Bacillus subtilis alpha-amylase, in complex with the pseudotetrasaccharide inhibitor acarbose, revealed an hexasaccharide in the active site as a result of transglycosylation. After comparison with the known structure of the catalytic-site mutant complexed with the native substrate maltopentaose, it is suggested that the present structure represents a mimic intermediate in the initial stage of the catalytic process. ...[more]
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