Ontology highlight
ABSTRACT:
SUBMITTER: Suzuki T
PROVIDER: S-EPMC2629239 | biostudies-literature | 2009 Jan
REPOSITORIES: biostudies-literature
Suzuki Takuya T Elias Bertha C BC Seth Ankur A Shen Le L Turner Jerrold R JR Giorgianni Francesco F Desiderio Dominic D Guntaka Ramareddy R Rao Radhakrishna R
Proceedings of the National Academy of Sciences of the United States of America 20081229 1
PKC eta is expressed predominantly in the epithelial tissues; however, its role in the regulation of epithelial tight junctions (TJs) is unknown. We present evidence that PKC eta phosphorylates occludin on threonine residues (T403 and T404) and plays a crucial role in the assembly and/or maintenance of TJs in Caco-2 and MDCK cell monolayers. Inhibition of PKC eta by specific pseudo substrate inhibitor or knockdown of PKC eta by specific shRNA disrupts the junctional distribution of occludin and ...[more]