Ontology highlight
ABSTRACT:
SUBMITTER: Sabariegos R
PROVIDER: S-EPMC2630637 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Sabariegos Rosario R Picazo Fernando F Domingo Beatriz B Franco Sandra S Martinez Miguel-Angel MA Llopis Juan J
Antimicrobial agents and chemotherapy 20081208 2
The NS3/4A protease from hepatitis C virus (HCV) plays a key role in viral replication. We report a system for monitoring the activity of this enzyme in single living mammalian cells. We constructed a fluorescence resonance energy transfer (FRET) probe that consists of an enhanced cyan fluorescent protein-citrine fusion, with a cleavage site for HCV NS3/4A protease embedded within the linker between them. Expression of the biosensor in mammalian cells resulted in a FRET signal, and cotransfectio ...[more]