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A dansyl fluorescence-based assay for monitoring kinetics of lipid extraction and transfer.

ABSTRACT: Lipid transfer proteins have important roles in cellular biology, and fluorescence spectroscopy has found wide range use as a facile means for time-resolved monitoring of protein-lipid interactions. Here, we show how the fluorescence emission properties of dansyl-DHPE can be exploited to characterize lipid extraction and lipid transfer kinetics. The GM2 activator protein serves as an example of a lipid transfer protein where the ability to independently characterize lipid extraction from donor vesicles, formation of a protein:lipid complex in solution, and release of lipid from the complex to acceptor liposomes is crucial for full kinetic characterization of lipid transfer.

SUBMITTER: Ran Y

PROVIDER: S-EPMC2633774 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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A dansyl fluorescence-based assay for monitoring kinetics of lipid extraction and transfer.

Ran Yong Y Fanucci Gail E GE

Analytical biochemistry 20080729 2

Lipid transfer proteins have important roles in cellular biology, and fluorescence spectroscopy has found wide range use as a facile means for time-resolved monitoring of protein-lipid interactions. Here, we show how the fluorescence emission properties of dansyl-DHPE can be exploited to characterize lipid extraction and lipid transfer kinetics. The GM2 activator protein serves as an example of a lipid transfer protein where the ability to independently characterize lipid extraction from donor v ...[more]

PMID: 18694718

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