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The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction.


ABSTRACT:

Background

The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes.

Methodology/principal findings

We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, performed in parallel with in vivo genetic experimentation, defined the HU regulon. This large regulon, which comprises 8% of the genome, is composed of four biologically relevant gene classes whose regulation responds to anaerobiosis, acid stress, high osmolarity, and SOS induction.

Conclusions/significance

The regulation a large number of genes encoding enzymes involved in energy metabolism and catabolism pathways by HU explains the highly pleiotropic phenotype of HU-deficient cells. The uniform chromosomal distribution of the many operons regulated by HU strongly suggests that the transcriptional and nucleoid architectural functions of HU constitute two aspects of a unique protein-DNA interaction mechanism.

SUBMITTER: Oberto J 

PROVIDER: S-EPMC2634741 | biostudies-literature | 2009

REPOSITORIES: biostudies-literature

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Publications

The HU regulon is composed of genes responding to anaerobiosis, acid stress, high osmolarity and SOS induction.

Oberto Jacques J   Nabti Sabrina S   Jooste Valérie V   Mignot Hervé H   Rouviere-Yaniv Josette J  

PloS one 20090204 2


<h4>Background</h4>The Escherichia coli heterodimeric HU protein is a small DNA-bending protein associated with the bacterial nucleoid. It can introduce negative supercoils into closed circular DNA in the presence of topoisomerase I. Cells lacking HU grow very poorly and display many phenotypes.<h4>Methodology/principal findings</h4>We analyzed the transcription profile of every Escherichia coli gene in the absence of one or both HU subunits. This genome-wide in silico transcriptomic approach, p  ...[more]

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