Dataset Information

Highly boron deficiency-tolerant plants generated by enhanced expression of NIP5;1, a boric acid channel.

ABSTRACT: Boron (B) is an essential element for plants, and B deficiency is a worldwide agricultural problem. In B-deficient areas, B is often supplied as fertilizer, but excess B can be toxic to both plants and animals. Generation of B deficiency-tolerant plants could reduce B fertilizer use. Improved fertility under B-limiting conditions in Arabidopsis thaliana by overexpression of BOR1, a B transporter, has been reported, but the root growth was not improved by the BOR1 overexpression. In this study, we report that enhanced expression of NIP5;1, a boric acid channel for efficient B uptake, resulted in improved root elongation under B-limiting conditions in A. thaliana. An NIP5;1 activation tag line, which has a T-DNA insertion with enhancer sequences near the NIP5;1 gene, showed improved root elongation under B limitation. We generated a construct which mimics the tag line: the cauliflower mosaic virus 35S RNA promoter was inserted at 1,357 bp upstream of the NIP5;1 transcription initiation site. Introduction of this construct into the nip5;1-1 mutant and the BOR1 overexpresser resulted in enhanced expression of NIP5;1 and improved root elongation under low B supply. Furthermore, one of the transgenic lines exhibited improved fertility and short-term B uptake. Our results demonstrate successful improvement of B deficiency tolerance and the potential of enhancing expression of a mineral nutrient channel gene to improve growth under nutrient-limiting conditions.

SUBMITTER: Kato Y

PROVIDER: S-EPMC2638715 | biostudies-literature | 2009 Jan

REPOSITORIES: biostudies-literature

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Highly boron deficiency-tolerant plants generated by enhanced expression of NIP5;1, a boric acid channel.

Kato Yuichi Y Miwa Kyoko K Takano Junpei J Wada Motoko M Fujiwara Toru T

Plant & cell physiology 20081117 1

Boron (B) is an essential element for plants, and B deficiency is a worldwide agricultural problem. In B-deficient areas, B is often supplied as fertilizer, but excess B can be toxic to both plants and animals. Generation of B deficiency-tolerant plants could reduce B fertilizer use. Improved fertility under B-limiting conditions in Arabidopsis thaliana by overexpression of BOR1, a B transporter, has been reported, but the root growth was not improved by the BOR1 overexpression. In this study, w ...[more]

PMID: 19017629

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Project description:The strong expansion potential of invasive plants is often attributed to fast adaptive responses to stress. However, the evolution of tolerance to one stressor may affect the responses to other stressors. Currently, it remains unclear what effect the evolution to one stressor might have on the responses to other single or combined stressors. Moreover, it is unknown how this might differ between invasive and native species.Invasive plants (Mikania micrantha and Bidens pilosa) and native plants (Merremia hederacea and Sida acuta) from low- and high-salinity habitats were grown under control and stressful conditions [salt stress, water stress (drought/waterlogging), and their combinations]. We explored the effects of evolved salt tolerance on the responses to water stress/combined stresses and the underlying trait mechanisms.The high-salinity populations of all species exhibited stronger salt tolerance than the low-salinity populations. As to the tolerance to other stressors, the high-salinity and low-salinity populations of the invasive species were similar, whereas the high-salinity populations of the native species exhibited stronger tolerance than the low-salinity populations under most stress treatments. However, the enhanced salt tolerance in native species was accompanied by reduced total biomass under control condition. The stress tolerance of native species correlated with leaf production rate and allocation to root, while the performance of native species under control condition correlated with leaf morphology and carbon assimilation rate. This suggests a trade-off between salt tolerance and performance in the native but not the invasive species, probably resulting from altered phenotypic/physiological traits. Synthesis:Our work suggests that the evolution of tolerance to one stressor may have stronger effects on the tolerance to other stressors of the native compared with the invasive species. This may be a new paradigm to explain the greater advantage of invasive vs. native species in highly stressful habitats.

Dataset Information

Highly boron deficiency-tolerant plants generated by enhanced expression of NIP5;1, a boric acid channel.

Publications

Highly boron deficiency-tolerant plants generated by enhanced expression of NIP5;1, a boric acid channel.

Similar Datasets

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