Unknown

Dataset Information

0

Unbalanced deoxynucleotide pools cause mitochondrial DNA instability in thymidine phosphorylase-deficient mice.


ABSTRACT: Replication and repair of DNA require equilibrated pools of deoxynucleoside triphosphate precursors. This concept has been proven by in vitro studies over many years, but in vivo models are required to demonstrate its relevance to multicellular organisms and to human diseases. Accordingly, we have generated thymidine phosphorylase (TP) and uridine phosphorylase (UP) double knockout (TP(-/-)UP(-/-)) mice, which show severe TP deficiency, increased thymidine and deoxyuridine in tissues and elevated mitochondrial deoxythymidine triphosphate. As consequences of the nucleotide pool imbalances, brains of mutant mice developed partial depletion of mtDNA, deficiencies of respiratory chain complexes and encephalopathy. These findings largely account for the pathogenesis of mitochondrial neurogastrointestinal encephalopathy (MNGIE), the first inherited human disorder of nucleoside metabolism associated with somatic DNA instability.

SUBMITTER: Lopez LC 

PROVIDER: S-EPMC2638828 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

altmetric image

Publications

Unbalanced deoxynucleotide pools cause mitochondrial DNA instability in thymidine phosphorylase-deficient mice.

López Luis C LC   Akman Hasan O HO   García-Cazorla Angeles A   Dorado Beatriz B   Martí Ramón R   Nishino Ichizo I   Tadesse Saba S   Pizzorno Giuseppe G   Shungu Dikoma D   Bonilla Eduardo E   Tanji Kurenai K   Hirano Michio M  

Human molecular genetics 20081121 4


Replication and repair of DNA require equilibrated pools of deoxynucleoside triphosphate precursors. This concept has been proven by in vitro studies over many years, but in vivo models are required to demonstrate its relevance to multicellular organisms and to human diseases. Accordingly, we have generated thymidine phosphorylase (TP) and uridine phosphorylase (UP) double knockout (TP(-/-)UP(-/-)) mice, which show severe TP deficiency, increased thymidine and deoxyuridine in tissues and elevate  ...[more]

Similar Datasets

| S-EPMC3115590 | biostudies-literature
| S-EPMC139774 | biostudies-literature
| S-EPMC6517976 | biostudies-literature
| S-EPMC9403154 | biostudies-literature
| S-EPMC2948953 | biostudies-literature
| S-EPMC3820243 | biostudies-literature
| S-EPMC3079504 | biostudies-literature
| S-EPMC8105268 | biostudies-literature
| S-EPMC6014165 | biostudies-literature
| S-EPMC4258140 | biostudies-literature