Estimation of errors introduced by confocal imaging into the data on segmentation gene expression in Drosophila.

ABSTRACT:

Motivation

Currently the confocal scanning microscopy of fluorescently tagged molecules is extensively employed to acquire quantitative data on gene expression at cellular resolution. Following this approach, we generated a large dataset on the expression of segmentation genes in the Drosophila blastoderm, that is widely used in systems biology studies. As data accuracy is of critical importance for the success of studies in this field, we took a shot to evaluate possible errors introduced in the data by acquisition and processing methods. This article deals with errors introduced by confocal microscope.

Results

In confocal imaging, the inevitable photon noise is commonly reduced by the averaging of multiple frames. The averaging may introduce errors into the data, if single

SUBMITTER: Myasnikova E 

PROVIDER: S-EPMC2639076 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature