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Efficient gene delivery vectors by tuning the surface charge density of amino acid-functionalized gold nanoparticles.

ABSTRACT: Gold colloids functionalized with amino acids provide a scaffold for effective DNA binding with subsequent condensation. Particles with lysine and lysine dendron functionality formed particularly compact complexes and provided highly efficient gene delivery without any observed cytotoxicity. Nanoparticles functionalized with first generation lysine dendrons (NP-LysG1) were approximately 28-fold superior to polylysine in reporter gene expression. These amino acid-based nanoparticles were responsive to intracellular glutathione levels, providing a tool for controlled release and concomitant expression of DNA.

SUBMITTER: Ghosh PS

PROVIDER: S-EPMC2643365 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Efficient gene delivery vectors by tuning the surface charge density of amino acid-functionalized gold nanoparticles.

Ghosh Partha S PS Kim Chae-Kyu CK Han Gang G Forbes Neil S NS Rotello Vincent M VM

ACS nano 20081101 11

Gold colloids functionalized with amino acids provide a scaffold for effective DNA binding with subsequent condensation. Particles with lysine and lysine dendron functionality formed particularly compact complexes and provided highly efficient gene delivery without any observed cytotoxicity. Nanoparticles functionalized with first generation lysine dendrons (NP-LysG1) were approximately 28-fold superior to polylysine in reporter gene expression. These amino acid-based nanoparticles were responsi ...[more]

PMID: 19206385

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