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Cytosine deaminase as a negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria.

ABSTRACT: We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors.

SUBMITTER: Dubeau MP

PROVIDER: S-EPMC2643560 | biostudies-literature | 2009 Feb

REPOSITORIES: biostudies-literature

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Cytosine deaminase as a negative selection marker for gene disruption and replacement in the genus Streptomyces and other actinobacteria.

Dubeau Marie-Pierre MP Ghinet Mariana Gabriela MG Jacques Pierre-Etienne PE Clermont Nancy N Beaulieu Carole C Brzezinski Ryszard R

Applied and environmental microbiology 20081219 4

We developed a novel negative selection system for actinobacteria based on cytosine deaminase (CodA). We constructed vectors that include a synthetic gene encoding the CodA protein from Escherichia coli optimized for expression in Streptomyces species. Gene disruption and the introduction of an unmarked in-frame deletion were successfully achieved with these vectors. ...[more]

PMID: 19098221

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