Ontology highlight
ABSTRACT:
SUBMITTER: Portman N
PROVIDER: S-EPMC2645819 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Portman Neil N Lacomble Sylvain S Thomas Benjamin B McKean Paul G PG Gull Keith K
The Journal of biological chemistry 20081211 9
Eukaryotic flagella from organisms such as Trypanosoma brucei can be isolated and their protein components identified by mass spectrometry. Here we used a comparative approach utilizing two-dimensional difference gel electrophoresis and isobaric tags for relative and absolute quantitation to reveal protein components of flagellar structures via ablation by inducible RNA interference mutation. By this approach we identified 20 novel components of the paraflagellar rod (PFR). Using epitope tagging ...[more]