New strategy for quantifying biological zinc by a modified zinpyr fluorescence sensor.
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ABSTRACT:
SUBMITTER: Zhang XA
PROVIDER: S-EPMC2645955 | biostudies-literature | 2008 Nov
REPOSITORIES: biostudies-literature
Publications
New strategy for quantifying biological zinc by a modified zinpyr fluorescence sensor.
Zhang Xiao-an XA Hayes Dugan D Smith Sarah J SJ Friedle Simone S Lippard Stephen J SJ
Journal of the American Chemical Society 20081101 47
Substitution of one pyridine by pyrazine in each DPA appendage of ZP1 leads to a new zinc sensor, ZPP1, with a modified background fluorescence and zinc affinity. ZPP1 exhibits a two-step zinc response during fluorescence titrations, which leads to a new method for zinc quantification. The ability of ZPP1 to image and quantify zinc was demonstrated in pancreatic Min6 cells. ...[more]
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