Annotating genomes with massive-scale RNA sequencing.
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ABSTRACT:
SUBMITTER: Denoeud F
PROVIDER: S-EPMC2646279 | biostudies-literature | 2008
REPOSITORIES: biostudies-literature
Publications
Annotating genomes with massive-scale RNA sequencing.
Denoeud France F Aury Jean-Marc JM Da Silva Corinne C Noel Benjamin B Rogier Odile O Delledonne Massimo M Morgante Michele M Valle Giorgio G Wincker Patrick P Scarpelli Claude C Jaillon Olivier O Artiguenave François F
Genome biology 20081216 12
Next generation technologies enable massive-scale cDNA sequencing (so-called RNA-Seq). Mainly because of the difficulty of aligning short reads on exon-exon junctions, no attempts have been made so far to use RNA-Seq for building gene models de novo, that is, in the absence of a set of known genes and/or splicing events. We present G-Mo.R-Se (Gene Modelling using RNA-Seq), an approach aimed at building gene models directly from RNA-Seq and demonstrate its utility on the grapevine genome. ...[more]
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