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Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels.

ABSTRACT: Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix format without interference from protein features.

SUBMITTER: Deb SK

PROVIDER: S-EPMC2646756 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature

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Detection and relative quantification of proteins by surface enhanced Raman using isotopic labels.

Deb Shirshendu K SK Davis Brandon B Knudsen Giselle M GM Gudihal Ravindra R Ben-Amotz Dor D Davisson V Jo VJ

Journal of the American Chemical Society 20080701 30

Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix for ...[more]

PMID: 18593116

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