Ontology highlight
ABSTRACT:
SUBMITTER: Deb SK
PROVIDER: S-EPMC2646756 | biostudies-literature | 2008 Jul
REPOSITORIES: biostudies-literature
Deb Shirshendu K SK Davis Brandon B Knudsen Giselle M GM Gudihal Ravindra R Ben-Amotz Dor D Davisson V Jo VJ
Journal of the American Chemical Society 20080701 30
Accurate quantification of protein content and composition has been achieved using isotope-edited surface enhanced resonance Raman spectroscopy. Synthesis of isotopomeric Rhodamine dye-linked bioconjugation reagents enabled direct labeling of surface lysines on a variety of proteins. When separated in polyacrylamide gels and stained with silver nanoparticles. The spectral signatures reflect the expected statistical distribution of isotopomeric labels on the labeled proteins in the gel matrix for ...[more]