Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy.
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ABSTRACT:
SUBMITTER: Davalos D
PROVIDER: S-EPMC2647134 | biostudies-literature | 2008 Mar
REPOSITORIES: biostudies-literature
Publications
Stable in vivo imaging of densely populated glia, axons and blood vessels in the mouse spinal cord using two-photon microscopy.
Davalos Dimitrios D Lee Jae K JK Smith W Bryan WB Brinkman Brendan B Ellisman Mark H MH Zheng Binhai B Akassoglou Katerina K
Journal of neuroscience methods 20071128 1
In vivo imaging has revolutionized our understanding of biological processes in brain physiology and pathology. However, breathing-induced movement artifacts have impeded the application of this powerful tool in studies of the living spinal cord. Here we describe in detail a method to image stably and repetitively, using two-photon microscopy, the living spinal tissue in mice with dense fluorescent cells or axons, without the need for animal intubation or image post-processing. This simplified t ...[more]
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