Ontology highlight
ABSTRACT:
SUBMITTER: Yoshimoto R
PROVIDER: S-EPMC2647322 | biostudies-literature | 2009 Feb
REPOSITORIES: biostudies-literature
Yoshimoto Rei R Kataoka Naoyuki N Okawa Katsuya K Ohno Mutsuhito M
Nucleic acids research 20081222 3
Pre-mRNA splicing occurs in a large complex spliceosome. The steps of both spliceosome assembly and splicing reaction have been extensively analyzed, and many of the factors involved have been identified. However, the post-splicing intron turnover process, especially in vertebrates, remains to be examined. In this paper, we developed a two-tag affinity purification method for purifying lariat intron RNA-protein complexes obtained from an in vitro splicing reaction. Glycerol gradient sedimentatio ...[more]