Multilocus sequence typing of Oenococcus oeni: detection of two subpopulations shaped by intergenic recombination.

ABSTRACT: Oenococcus oeni is the acidophilic lactic acid bacterial species most frequently associated with malolactic fermentation of wine. Since the description of the species (formerly Leuconostoc oenos), characterization of indigenous strains and industrially produced cultures by diverse typing methods has led to divergent conclusions concerning the genetic diversity of strains. In the present study, a multilocus sequence typing (MLST) scheme based on the analysis of eight housekeeping genes was developed and tested on a collection of 43 strains of diverse origins. The eight targeted loci were successfully amplified and sequenced for all isolates. Only three to 11 different alleles were detected for these genes. The average nucleotide diversity also was rather limited (0.0011 to 0.0370). Despite

SUBMITTER: Bilhere E 

PROVIDER: S-EPMC2648160 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature