Comparison of the genotyping results using DNA obtained from blood and saliva.

ABSTRACT:

Aim

Traditionally, large scale genotyping projects have used DNA derived from whole-blood or lymphoblast cell lines. But over the past several years, a number of investigators have begun to use DNA prepared from saliva for genotyping studies, particularly for use in behavioral genetic studies. However, the comparability of DNA from these two sources has not been rigorously analyzed by unbiased sources.

Objective

In this communication, we compare the single nucleotide polymorphism genotyping results from DNA derived from whole-blood samples obtained from 474 participants from the Iowa Adoption Studies with that of saliva samples prepared from 555 members of the Strong African-American Families project.

Results

We found that DNA prepared from whole-blood performed signi

SUBMITTER: Philibert RA 

PROVIDER: S-EPMC2648613 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature