Unknown

Dataset Information

0

Cyclin D1-cdk4 induce runx2 ubiquitination and degradation.


ABSTRACT: Runx2 is a Runt domain transcription factor involved in the activation of genes encoding osteoblast and chondrocyte-specific proteins. Runx2 activity is regulated by transcriptional and post-transcriptional mechanisms. The functional significance of the post-translational modification of Runx2 has not been fully defined. We show that cyclin D1-Cdk4 induce Runx2 degradation in an ubiquitination-proteasome-dependent manner. Mutagenesis of Runx2 serine-472, a consensus Cdk site, to alanine increases the half-life of Runx2 and causes loss of sensitivity to cyclin D1-induced Runx2 degradation. The targeted Runx2 degradation by cyclin D1 identifies a novel mechanism through which Runx2 activity is regulated coordinately with the cell cycle machinery in bone cells.

SUBMITTER: Shen R 

PROVIDER: S-EPMC2649830 | biostudies-literature | 2006 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications

Cyclin D1-cdk4 induce runx2 ubiquitination and degradation.

Shen Run R   Wang Xiumei X   Drissi Hicham H   Liu Fang F   O'Keefe Regis J RJ   Chen Di D  

The Journal of biological chemistry 20060413 24


Runx2 is a Runt domain transcription factor involved in the activation of genes encoding osteoblast and chondrocyte-specific proteins. Runx2 activity is regulated by transcriptional and post-transcriptional mechanisms. The functional significance of the post-translational modification of Runx2 has not been fully defined. We show that cyclin D1-Cdk4 induce Runx2 degradation in an ubiquitination-proteasome-dependent manner. Mutagenesis of Runx2 serine-472, a consensus Cdk site, to alanine increase  ...[more]

Similar Datasets

| S-EPMC2671927 | biostudies-literature
| S-EPMC3185142 | biostudies-literature
| S-EPMC2722223 | biostudies-literature
| S-EPMC10491536 | biostudies-literature
| S-EPMC5297679 | biostudies-literature
| S-EPMC2856324 | biostudies-literature
| S-EPMC6690700 | biostudies-literature
| S-EPMC1702390 | biostudies-literature
| S-EPMC8395054 | biostudies-literature
| S-EPMC4614005 | biostudies-literature