Unknown

Dataset Information

0

Purification, crystallization and preliminary crystallographic study of low oxygen-affinity haemoglobin from cat (Felis silvestris catus) in two different crystal forms.

ABSTRACT: Haemoglobin is a metalloprotein which plays a major role in the transportation of oxygen from the lungs to tissues and of carbon dioxide back to the lungs. The present work reports the preliminary crystallographic study of low oxygen-affinity haemoglobin from cat in different crystal forms. Cat blood was collected, purified by anion-exchange chromatography and crystallized in two different conditions by the hanging-drop vapour-diffusion method under unbuffered low-salt and buffered high-salt concentrations using PEG 3350 as a precipitant. Intensity data were collected using MAR345 and MAR345dtb image-plate detector systems. Cat haemoglobin crystallizes in monoclinic and orthorhombic crystal forms with one and two whole biological molecules (alpha(2)beta(2)), respectively, in the asymmetric unit.

SUBMITTER: Balasubramanian M 

PROVIDER: S-EPMC2650469 | biostudies-literature | 2009 Mar

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Purification, crystallization and preliminary crystallographic study of low oxygen-affinity haemoglobin from cat (Felis silvestris catus) in two different crystal forms.

Balasubramanian M M   Moorthy Pon Sathya PS   Neelagandan K K   Ponnuswamy M N MN  

Acta crystallographica. Section F, Structural biology and crystallization communications 20090226 Pt 3

Haemoglobin is a metalloprotein which plays a major role in the transportation of oxygen from the lungs to tissues and of carbon dioxide back to the lungs. The present work reports the preliminary crystallographic study of low oxygen-affinity haemoglobin from cat in different crystal forms. Cat blood was collected, purified by anion-exchange chromatography and crystallized in two different conditions by the hanging-drop vapour-diffusion method under unbuffered low-salt and buffered high-salt con  ...[more]

Similar Datasets

Unknown A suite of genetic markers useful in assessing wildcat (Felis silvestris ssp.)-domestic cat (Felis silvestris catus) admixture.
| S-EPMC3157884 | biostudies-literature
Unknown When domestic cat (Felis silvestris catus) population structures interact with their viruses.
| S-EPMC7185750 | biostudies-literature
Unknown An autosomal genetic linkage map of the domestic cat, Felis silvestris catus.
| S-EPMC2656606 | biostudies-literature
Unknown Hybridization between Felis silvestris silvestris and Felis silvestris catus in two contrasted environments in France.
| S-EPMC6972816 | biostudies-literature
Unknown Extent of linkage disequilibrium in the domestic cat, Felis silvestris catus, and its breeds.
| S-EPMC3538540 | biostudies-literature
Unknown Standardization of a SNP panel for parentage verification and identification in the domestic cat (Felis silvestris catus).
| S-EPMC8519126 | biostudies-literature
Unknown Purification, crystallization and preliminary crystallographic study of haemoglobin from camel (Camelus dromedarius): a high oxygen-affinity lowland species.
| S-EPMC2720330 | biostudies-literature
Unknown Annotated features of domestic cat - Felis catus genome.
| S-EPMC4138527 | biostudies-literature
Unknown The genetic integrity of the ex situ population of the European wildcat (Felis silvestris silvestris) is seriously threatened by introgression from domestic cats (Felis silvestris catus).
| S-EPMC4146591 | biostudies-literature
Unknown An assessment of the role of the falx cerebri and tentorium cerebelli in the cranium of the cat (Felis silvestris catus).
| S-EPMC6228496 | biostudies-literature