Dataset Information

Genome-wide analysis of Chlamydophila pneumoniae gene expression at the late stage of infection.

ABSTRACT: Chlamydophila pneumoniae, an obligate intracellular eubacterium, changes its form from a vegetative reticulate body into an infectious elementary body during the late stage of its infection cycle. Comprehension of the molecular events in the morphological change is important to understand the switching mechanism between acute and chronic infection, which is deemed to relate to the pathogenesis of atherosclerosis. Herein, we have attempted to screen genes expressed in the late stage with a genome-wide DNA microarray, resulting in nomination of 17 genes as the late-stage genes. Fourteen of the 17 genes and six other genes predicted as late-stage genes were confirmed to be up-regulated in the late stage with a quantitative reverse transcriptase-polymerase chain reaction. These 20 late-stage genes were classified into two groups by clustering analysis: 'drastically induced' and 'moderately induced' genes. Out of eight drastically induced genes, four contain sigma(28) promoter-like sequences and the other four contain an upstream common sequence. It suggests that besides sigma(28), there are certain up-regulatory mechanisms at the late stage, which may be involved in the chlamydial morphological change and thus pathogenesis.

SUBMITTER: Miura K

PROVIDER: S-EPMC2650627 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature

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Genome-wide analysis of Chlamydophila pneumoniae gene expression at the late stage of infection.

Miura Koshiro K Toh Hidehiro H Hirakawa Hideki H Sugii Manabu M Murata Masayuki M Nakai Kenta K Tashiro Kosuke K Kuhara Satoru S Azuma Yoshinao Y Shirai Mutsunori M

DNA research : an international journal for rapid publication of reports on genes and genomes 20080124 2

Chlamydophila pneumoniae, an obligate intracellular eubacterium, changes its form from a vegetative reticulate body into an infectious elementary body during the late stage of its infection cycle. Comprehension of the molecular events in the morphological change is important to understand the switching mechanism between acute and chronic infection, which is deemed to relate to the pathogenesis of atherosclerosis. Herein, we have attempted to screen genes expressed in the late stage with a genome ...[more]

PMID: 18222926

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Project description:The obligate intracellular, gram-negative bacterium, Chlamydophila pneumoniae (Cpn), has impact as an acute and chronic disease-causing pathogen. Little is known about changes in the Cpn transcriptome during its biphasic developmental cycle (the acute infection) and persistence stages that are linked to chronic diseases. To analyze Cpn CWL029 gene expression, we designed a pathogen-specific oligo microarray and optimized the extraction method for pathogen RNA. Throughout the acute infection, ratio expression profiles for each gene were generated using 48 hours post infection as a reference. Based on these profiles, significantly expressed genes were separated into 12 expression clusters using SOM clustering and manually into the ‘early’, ‘mid’, ‘late’ and ‘tardy’ cluster classes. The latter two were differentiated because the ‘tardy’ class showed steadily increasing expression at the end of the cycle. The transcriptome of the Cpn elementary body (EB) and published EB proteomics data were compared to the cluster profile of the acute infection. We found an intriguing association between ‘late’ genes and genes coding for EB proteins, whereas ‘tardy’ genes were mainly associated with genes coding for EB mRNA. It has been published that iron depletion leads to Cpn persistence. We compared the gene expression profiles during iron depletion-mediated persistence with the expression clusters of the acute infection. This led to the finding that establishment of iron depletion-mediated persistence is more likely a mid-cycle arrest in development rather than a completely distinct gene expression pattern. Taken together, here we describe the Cpn transcriptome during the acute infection with ‘late’ genes correlating to EB proteins and ‘tardy’ genes to EB mRNA. Expression profiles during iron mediated-persistence led us to propose the hypothesis that the transcriptomic ‘clock’ is arrested during acute mid-cycle. Keywords: time course, iron, persistence, Chlamydia, Chlamydophila, pneumoniae, recovery, pathogen, life cycle, developmental cycle

Dataset Information

Genome-wide analysis of Chlamydophila pneumoniae gene expression at the late stage of infection.

Publications

Genome-wide analysis of Chlamydophila pneumoniae gene expression at the late stage of infection.

Similar Datasets

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